What BPC-157 is
BPC-157, also written BPC157 and often searched as "BPC 157 peptide", is a synthetic pentadecapeptide, fifteen amino acids long, with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val. It was first described by Predrag Sikirić and colleagues at the University of Zagreb as a partial sequence of a protein isolated from human gastric juice, which they called Body Protection Compound; the number refers to the fragment they synthesised and studied. It has no disulfide bridge, no lipid and no N- or C-terminal modification, and it is unusually stable in acidic conditions, a property the original authors attributed to its origin in the stomach.
The molecule is notable for how much published work exists on so simple a sequence. It is small, hydrophilic and free of the oxidation-prone residues that complicate the analysis of longer peptides. The average molecular weight is approximately 1,419 g/mol and the CAS number is 137525-51-0. The published literature on the compound, with references, is collected in the monograph.
What you receive
Each vial contains BPC-157 as a white lyophilised powder, sealed with a flip-off cap over a crimped stopper, in the net quantity stated on the label: 5 mg or 10 mg. There is no carrier, mannitol or other excipient unless the batch certificate says otherwise. The batch number is printed on the label and matches the certificate in our register.
Reading the certificate for a BPC-157 batch
Purity by reversed-phase HPLC is reported as the area of the main peak against all peaks and should be above 99% for a peptide this short; anything much lower suggests a poorly executed synthesis. Identity by LC-MS is reported as the observed mass against the expected mass of about 1,419 Da. For a peptide with no modifications the mass check is straightforward, and the main thing it rules out is a deletion sequence missing one of the five proline or two aspartate residues, which would be visible as a mass difference of around 97 or 115 daltons respectively. Net peptide content gives the milligrams of peptide actually present after allowing for water and the acetate or arginate counter-ion.
The impurities seen with BPC-157 are usually deletion sequences and a small amount of aspartimide-related product from the Asp-Asp pair, both of which elute close to the main peak. At the sub-1% level these are normal. Our guide to reading a certificate of analysis shows annotated examples, and HPLC and LC-MS explained describes the methods.
Storage and handling
Sealed vials should be kept refrigerated at 2 to 8 °C and protected from light; −20 °C is preferable for storage beyond a few months, and the lyophilised powder is stable for well over a year under those conditions. BPC-157 is less hygroscopic than the lipidated peptides, but it is still good practice to allow the vial to reach room temperature before opening. The peptide dissolves readily in water, saline or bacteriostatic water and is stable in solution at refrigerator temperature for longer than most peptides, a property documented in the original literature; it also tolerates mildly acidic conditions. The storage and stability guide covers solvents, concentrations and aliquoting.